LRP1 Receptor Controls Adipogenesis and Is Up-Regulated In Human and Mouse Obese Adipose Tissue
Figure 3
Inhibition of LRP1 expression by silencing during adipocyte differentiation.
(A) Silencing of LRP1 by siRNAs. 3T3F442A preadipocytes were transiently transfected with Luc siRNA or LRP1 siRNAs. LRP1β protein expression was monitored by immunoblotting 2 days post-transfection. α tubulin was used as a loading control. A representative experiment out of 3 is shown. (B) Time-course of LRP1β protein expression after Luc siRNA or LRP1 siRNAs transfection during adipogenesis. 3T3F442A preadipocytes were transiently transfected with Luc siRNA or LRP1 siRNAs. Two-days post-transfection, LRP1β protein expression was monitored by immunoblotting 0, 3, 5 or 7 days after differentiation induction. ERK2 was used as a loading control.