Copper-Triggered Aggregation of Ubiquitin
Figure 5
CuII and TFE induced conformational change of Ub.
A) Far-UV CD spectra of Ub incubated at 37°C with 3 mol equiv of CuII in 20% TFE for different time intervals: 1 h (blue line); 5 d (red); 1 mo (green); 2 mo (black); B) ATR-FTIR spectrum (absorbance at the top and second derivative at the bottom) in the amide I region of native Ub (blue line) and of Ub incubated with 3 mol equiv of CuII in 20% TFE for 1 w (red) and 2 mo (black).