Loss of the Putative Catalytic Domain of HDAC4 Leads to Reduced Thermal Nociception and Seizures while Allowing Normal Bone Development
Figure 1
A. Diagram of the mutated HDAC4 locus. Genotyping and RT-PCR primers are indicated by arrows, as described in the methods. Primers in Exons 14 and 16 are used for RT-PCR to assay the endogenous transcript in wildtype and mutant mice. Primers in the LTR and intron (solid line) are used to genotype the wildtype and mutant mice. B. RT-PCR analysis of wildtype (+/+) and HDAC4ΔC (−/−) mice. Kidney and spleen tissue was analyzed from one +/+ and one −/− mice. Each sample includes a negative control without Reverse Transcriptase, an assay with Hdac4 primers alone, and an assay that includes an internal positive control for Actin. C. Protein sequence of mouse HDAC4. Bold letters correspond to histone deacetylase (HDAC) domain. Underlined amino acid sequence (aa 528-629) is equivalent to the region of human HDAC4 antigen (human aa. 530-631) of Santa Cruz antibody. Asterisk * denotes the site of retroviral insertion, which would terminate protein translation and putatively produce a partial protein of 1-747aa with molecular weight of 83.3 kDa.