Several Nuclear Events during Apoptosis Depend on Caspase-3 Activation but Do Not Constitute a Common Pathway
Figure 7
Neither HMGB1 nor CAD immobilization onto apoptotic chromatin depend on caspase-6 expression.
(A) Western blot of living (L) and apoptotic (A) MCF-7/pv and MCF-7/c3 cells. Caspase-7, but not caspase-6, is activated in MCF-7/pv forced to undergo apoptosis using TNF-α and CHX. Both caspases are activated in apoptotic MCF-7/c3 cells. (B) HeLa cells were stably transfected with Casp6shRNA or with the empty vector (evshRNA). Western blots show that caspase-6 expression is severely reduced in casp6shRNA HeLa cells. (C) FRAP experiments were performed in living and apoptotic evshRNA and casp6shRNA HeLa cells transiently transfected with HMGB1-GFP. The results are expressed as the mean +/− standard deviation (n = 20). (D) FRAP experiments were performed in living and apoptotic evshRNA and casp6shRNA HeLa cells transiently transfected with CAD-GFP. The results are expressed as the mean +/− standard deviation (n = 20).