Genetic and Proteomic Evidence for Roles of Drosophila SUMO in Cell Cycle Control, Ras Signaling, and Early Pattern Formation
Figure 5
Defects in the coordination of centrosome replication and spindle attachment in sumo GLC embryos.
A–B) Syncytial blastoderm Ore-R embryos. C) Uncoupled chromosome and centrosome replication observed in sumo04493 GLC embryos. D–J) In sumo04493 GLC embryos, multiple defects in metaphase spindle morphology are observed, including examples of anastral (arrow in F), monoastral (H), multipolar (E, G, arrows), as well as unfocused mitotic spindles (D′ and arrows in I, J). The embryos were stained for DAPI (blue), centrosomin (CNN, green), and α-Tubulin (red).