Complex Evolution of a Y-Chromosomal Double Homeobox 4 (DUX4)-Related Gene Family in Hominoids
Figure 1
Chromosomal organization of the human Y-chromosomal DUX locus with respect to the centromere-telomere orientation.
(A). The bar labeled on the left side with RP11-886I11 (AC134882 [8]) comprises the complete human DUXY locus. Only the BAC section harboring the locus is shown. The position of each DUXY gene is indicated by a red vertical line. Vertical dotted lines depict NcoI restriction sites. Each DUXY gene is represented by a characteristic restriction fragment. The size of each fragment is given below the corresponding DUXY gene. (B). Southern blot hybridization of the 281-bp DUXY PCR fragment to NcoI-digested DUXY BAC and cosmid DNAs. Along the left the DUXY genes giving rise to each fragment are listed. According to the hybridizing fragments the overlap of the DUXY cosmids can be determined (C). Cosmid contig of the human DUXY locus. Cosmids are positioned along the NcoI restriction map with respect to their DUXY content. The library address of each cosmid is indicated.