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Kinetochore-Independent Chromosome Poleward Movement during Anaphase of Meiosis II in Mouse Eggs

Figure 2

Time-lapse 3D confocal observation of chromosome poleward movements during anaphase of meiosis II.

(A) Movement of DNA beads (top) and meiotic chromosomes (lower) at different time points shown in minute in a timelapse 3D movie. M chromosomes represent maternal chromosomes. DNA beads and meiotic chromosomes are shown in green and spindle in red. Note that the apparent round morphology of DNA-bead spindle shown at 48 min was due to a slight spindle rotation in the Z dimension. (B) Entire oocyte showing synchronous anaphase events (including anaphase onset, chromosome poleward movement, spindle rotation, and polar body extrusion) in both the DNA bead-spindle (arrows) and the meiotic spindle (arrowheads).

Figure 2

doi: https://doi.org/10.1371/journal.pone.0005249.g002