Conservation of Carbohydrate Binding Interfaces — Evidence of Human HBGA Selection in Norovirus Evolution
Figure 4
Saliva-based binding results of various mutant P particles of Boxer (GI-8) with single amino acid changes at the three GI conserved sites (upper panel), at the regions corresponding to the three GII-conserved sites (middle panel), and at regions away from the predicted binding interface (lower panel).
The X-axes show the protein concentrations of the P particles and the Y axes indicate the optical densities at 450 nm (OD450) that were the average value of triplicate experiments. “O” and “A” represent the saliva samples of type O (containing H antigen) and A secretor, respectively, while “N” one of nonsecretor.