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The Signal Peptide of Staphylococcus aureus Panton Valentine Leukocidin LukS Component Mediates Increased Adhesion to Heparan Sulfates

Figure 2

Binding capacity of isogenic strains to ECM components.

Isogenic strains of Staphylococcus aureus examined for their binding capacity (Y-axis, absorbance reading at 540 nm) to collagen IV (Sigma) coated on 96-well plates, at concentrations of 5 µg/mL (identical results were obtained at concentrations of 50 µg/mL). Collagen I (white bars); collagen IV (grey bars); collagen V (dark grey bars); elastin (black bars), laminin (dotted bars). Parental (LUG960) indicates the genetic background in which all genetic modifications were made as follows: no insert plasmid, plasmid vector with no insert; phiSLT, lysogenization with phiSLT; Luk-PV plasmid, plasmid encoding for lukS-PV and lukF-PV; LukS-PV plasmid, plasmid encoding for lukS-PV; LukF-PV, plasmid encoding lukF-PV; chromosomal partial ΔPVL, partial deletion of the chromosomal luk-PV operon; 41aa Nterm LukS-PV, plasmid encoding for the first 41 amino acids of premature LukS-PV; 128aa Cterm LukF-PV, plasmid encoding for the last 128 amino acids of premature LukF-PV; LukS-PV SP, plasmid encoding the LukS-PV signal peptide; LukS-PV ΔSP, plasmid encoding LukS-PV without signal peptide; luk-PV mRNA, plasmid encoding for the luk-PV operon mRNA devoid of start codon; LukF-PV SP, plasmid encoding the LukF-PV signal peptide; TSST-1 SP, plasmid encoding the TSST-1 signal peptide; LukE-SP, plasmid encoding for the LukE signal peptide; LukD-SP; plasmid encoding for the LukD signal peptide. The vertical lines indicate the standard deviation.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0005042.g002