Identification of Coevolving Residues and Coevolution Potentials Emphasizing Structure, Bond Formation and Catalytic Coordination in Protein Evolution
Figure 9
Comparison of ZRes to other measures of coevolution.
(A) To ease processing load, calculations were limited to the 424 alignments with representative structures for which the product of the protein sequence length and alignment size was less than or equal to 100,000. Following the analysis performed previously [5], all residue pairs were ranked from highest to lowest ZRes score. For ranks 1 up to 100, the fraction of residue pairs at or higher than each rank lying within 6 Å of each other was calculated. The average of this contact accuracy across all alignments was then plotted (blue). The process was repeated with the Res (green), OMES (brown), McBASC (magenta), MIp (red), and MI (black) measures. (B) as in A, but utilizing all 1240 alignments with representative crystal structure. The results from one randomization of residue pair rankings are plotted in black. Statistical significance was assessed by Friedman's nonparametric 2-way ANOVA for measure effects on selectivity after factoring out rank effects. All pair-wise comparison in both A and B were significant except between MIp and Res.