The Warburg Effect Suppresses Oxidative Stress Induced Apoptosis in a Yeast Model for Cancer
Figure 3
Forced enhancement of respiration triggers cell death, suppresses growth, and elevates ROS production.
(A) Clonogenic assay of cultures pre-grown in liquid SCGlu. 500 cells were plated on SCGlu, SCGal, and SCGly agar plates, respectively (mean±SEM, n = 3; ***p<0.001). (B) Clonogenic assay of cultures pre-grown in liquid SCGly. 500 cells were plated on SCGly and SCGlu media plates, respectively (mean±SEM, n = 2; **p<0.01). (C) Cells from the whole colony (3 days) as well as from the central region (5 days) were stained for reactive oxygen species (ROS) with dihydroethidium (DHE). Values of relative fluorescent units (RFU) were normalized to wild-type strain values (mean±SEM, n = 2; **p<0.01, ***p<0.001). (D) Size of isolated wild-type colonies grown on SCGlu and SCGal media plates were monitored by taking photos at indicated time-points. Diameters were evaluated by processing the photos with Metamorph Imaging (white bar represents 1 cm) (mean±SEM, n = 3; *p<0.05, **p<0.01).