In-Vitro Helix Opening of M. tuberculosis oriC by DnaA Occurs at Precise Location and Is Inhibited by IciA Like Protein
Figure 4
ATP hydrolysis is essential for helix opening by rDnaA.
Helix opening assay was carried out as described earlier with SeqOriR1 as primer and the primer extension products were resolved on 6% sequencing gel. The reaction was carried out using 0.75 μg of rDnaA and 5 mM ATP (lane 1), 5 mM ADP (lane 2) and 5 mM ATP γ S (lane 3). 100 bp DNA ladder was used as a DNA molecular size marker (lane Mr). The markers on the right represent the size of the extension products.