In C. elegans, High Levels of dsRNA Allow RNAi in the Absence of RDE-4
Figure 7
X-cluster endo-siRNAs are more abundant in worms cultivated at lower temperatures.
(A) Northern analysis of total RNA isolated from adult worms grown at 16 or 25°C. The X-cluster endo-siRNAs (top panel), loading control (U6, bottom panel) and miRNA (let-7, bottom panel) were detected using end-labeled DNA oligonucleotide probes. (B) Northern analyses as in (A) were quantified, and the bar height shows the ratio of X-cluster siRNA to U6 at various temperatures (relative to 16°C); error bars indicate the SEM (n = 3). Data are not shown for adr;rde-4 and adr;rde-1;rde-4 strains because X-cluster endo-siRNAs were undetectable in these strains at any of the temperatures monitored (Fig. 7A).