Rickettsia conorii Transcriptional Response within Inoculation Eschar
Figure 1
Inoculation eschar from a patient with MSF.
(A) Histologic examination of inoculation eschar from a patient with MSF showing numerous dermal inflammatory infiltrates mainly composed of polymorphonuclear leukocytes with large necrotic areas and coagulative necrosis of the epidermis (hematoxylin-eosin-saffron, original magnification ×100). (B) Immunohistochemical detection of R. conorii in the inoculation eschar. Anti-R. conorii antibodies were detected using biotinylated secondary antibody, followed by avidin-peroxidase color development. The bacteria thus stained in reddish/brown, and indicated by the arrows, appear located between the necrotic inflammatory cells present in the dermis (original magnification ×400).