Induction of IgG3 to LPS via Toll-Like Receptor 4 Co-Stimulation
Figure 2
Natural Anti-LPS IgG3 is generated via MyD88-dependant TLR4 signaling.
A. Purified B cells from NOD P712H and NODWT mice were activated in vitro with LPS, CpG or αIgM and the proliferative response was assayed. ** P<0.01 when compared to NODWT mice. B. Total IgG and IgG1 vs. IgG3 antibodies to LPS in NODWT (n = 13) and NOD P712H mice (n = 7). *** P<0.001 when compared to NODWT mice. C. IgG antibodies to GAD, histone IIA, dsDNA and ssDNA in NODWT (n = 13) and NOD P712H mice (n = 7). D. IgG3 antibodies to LPS in WT, TLR2−/− and MyD88−/− mice (n = 4). *** P<0.001 when compared to WT or TLR2−/− mice. Results represent mean (±SD) of each group.