The Long March: A Sample Preparation Technique that Enhances Contig Length and Coverage by High-Throughput Short-Read Sequencing
Figure 1
Iterative rounds of GsuI digestion and barcoded adapter ligation create nested sub-libraries.
Adapter flanked cDNA molecules are attached to streptavidin beads via biotin modification of the Sol-S adapter. Yellow triangles indicate the GsuI recognition motif engineered into the Sol-L adapter, while the connected black arrow represents the distal cut site. Adapter barcodes and corresponding reads are classified as AA (green), CC (red), or TT (blue). Reads from the initial library and all three long march steps are aligned to form an 84 bp contig.