Human Keratinocytes Are Vanilloid Resistant
Figure 6
Fluorometric Ca2+ imaging showed no capsaicin-induced Ca-signal in keratinocytes.
Primary human keratinocytes and HaCaT cells were loaded with Fluo4-AM Ca2+-indicator then imaged in an appropriately equipped fluorescence microscope setup. The bars represent the increase in fluorescence upon capsaicin (2 µM) treatment as a % of the fluorescence increase after ionomycin treatment (50 µM). While TRPV1 transformed cells exposed to CAP showed readily detectable fluorescence changes, neither untransformed HaCaT cells, nor primary keratinocytes responded to this algesic vanilloid substance. Values are shown as means±SD for 6 measurement. The significance of differences compared with TRPV1ε/HaCaT was determined with the paired t-test, *P<0.05.