In Vitro Cellular Adaptations of Indicators of Longevity in Response to Treatment with Serum Collected from Humans on Calorie Restricted Diets
Figure 1
Proliferation of HepG2 cells cultured with serum from FEAST and CALERIE participants.
HepG2 cells were cultured in 96 well culture plates in culture media supplemented with 10% human serum. After 48 hours in culture, differences in the proliferation of HepG2 cells in serum collected at day 21 from FEAST participants or after 3 months from CALERIE participants were compared to the proliferation of cells cultured in serum from the same participants collected on baseline day. Graph shows % proliferation relative to baseline of cells cultured in serum collected from participants of the (A) FEAST study (n = 11) and (B) CALERIE (n = 9 for C group, n = 11 for CR group, n = 11 for CREX group) study. * = statistically significant p = 0.032).