The Difficult Case of Crystallization and Structure Solution for the ParC55 Breakage-Reunion Domain of Topoisomerase IV from Streptococcus pneumoniae
Figure 1
Analysis of purified ParC55 and its cysteine mutants by 10% SDS-polyacrylamide gel electrophoresis.
Lane 1, molecular-weight markers; lane 2, wild-type; lane 3, Cys109; Lane 4, Cys110; Lane 5, Cys190; lane 6, Cys294; lane 7, Cys387; lane 8, Cys426; lane 9, Cys437. The molecular weights of the markers are indicated.