Neutralization of Botulinum Neurotoxin by a Human Monoclonal Antibody Specific for the Catalytic Light Chain
Figure 3
Inhibition of the catalytic activity of the BoNT/A metalloprotease by the 4LCA antibody in vitro.
In this fluorescence resonance energy transfer assay (FRET), cleavage of the SNAPtide by BoNT/A LC un-quenches FITC fluorescence, which is quantified as arbitrary fluorescence units. In triplicate samples, the SNAPtide was incubated with BoNT/A alone or with the 6A or 4LCA antibodies and fluorescence was measured. Standard deviation (s.d.) is indicated by error bars.