Generation and Validation of a Shewanella oneidensis MR-1 Clone Set for Protein Expression and Phage Display
Figure 5
Briefly, phages were incubated with immobilized anti-E. coli thioredoxin antibody, non-binders were removed by washing, bound phage were eluted, amplified, and the process was repeated. Eluted phage clones from round 3 were DNA sequenced.