Deciphering the Ubiquitin-Mediated Pathway in Apicomplexan Parasites: A Potential Strategy to Interfere with Parasite Virulence
Figure 7
Domain architecture of RING and RING-like E3 ligases in P. falciparum compared to S. cerevisiae.
Domain architectures were retrieved using batch access to SMART database (http://smart.embl-heidelberg.de/smart/batch.pl). Domains in black and grey were retrieved from the Pfam database (http://pfam.sanger.ac.uk). Transmembrane segments are predicted with the TMHMM2 program, coiled coil regions are predicted with the Coils2 program, and signal peptides are predicted with the SignalP program. Legend: RING = RING finger; RINGv = C4HC3 RING-variant; IBR = in between RING fingers; ZnF_UBP = Ubiquitin Carboxyl-terminal Hydrolase-like zinc finger; ZnF_UBR1 = Putative zinc finger in N-recognin, a recognition component of the N-end rule pathway; PHD = plant homeodomain zinc finger; ZnF_NFX = zinc finger domain repressor of transcription; ZnF_C3H1 = zinc finger domain; ZnF_C2H2 = zinc finger domain; DEXDc = DEAD-like helicases superfamily; HELICc = helicase superfamily c-terminal domain; R3H = Putative single-stranded nucleic acids-binding domain; FYVE = zinc finger present in Fab1, YOTB, Vac1, and EEA1; WD40 = WD40 repeats; RRM = RNA recognition motif; FHA = Forkhead associated domain; SAP = Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation; Pfam MAT1 = CDK-activating kinase assembly factor MAT1; Pfam BRE1 = CDK-activating kinase assembly factor MAT1; Pfam HIRAN = HIP116, Rad5p N-terminal domain, found in the N-terminal regions of the SWI2/SNF2 proteins; Pfam BRAP2 = BRCA1-associated protein 2; Pfam Pex2-Pex12 = Pex2/Pex12 amino terminal region; Pfam ClpS = ATP-dependent Clp protease adaptor protein ClpS; Pfam SPX = SYG1, Pho81 and XPR1 domain; Pfam zf-UBR = Putative zinc finger in N-recognin (UBR box); Pfam zf-MIZ = MIZ/SP-RING zinc finger. The grey bar represents the primary structure of proteins.