Heparanase Facilitates Cell Adhesion and Spreading by Clustering of Cell Surface Heparan Sulfate Proteoglycans
Figure 5
Cell spreading induced by heparanase is mediated by PKC, Rac, and Src.
CHO cells were plated on the 110 kDa fibronectin-like protein, without (Con) or with heparanase, in the absence (Hepa) or presence of selective PKC (Bis), Src (PP2), ROCK (Y27632), MAPK (PD), PKA (H89), and Rac (NSC 23766) inhibitors for 6 hours. Cells were then fixed with 4% paraformaldehyde and stained with phalloidin-TRITC.