Activation of Estrogen Receptor-α by E2 or EGF Induces Temporally Distinct Patterns of Large-Scale Chromatin Modification and mRNA Transcription
Figure 7
Temporal single-cell analyses of ER-S118A and ER-S118E transcriptional response at the PRL-array.
PRL-HeLa cells transiently expressing the two phosphomutants, GFP-ERS118A (panel A and C) or GFP-ERS118E (panel B and D), were treated with either ethanol, E2 (panel A and B) or EGF (panel C and D) for different times. Subsequent to ligand treatment, the cells were fixed and subjected to RNA FISH using a biotinylated dsRED2 probe followed by fluorescent-tagged streptavidin. The FISH signal at the array was determined as described and the value graphed as fold induction over vehicle control cells (solid line). For each cell the area of the array was also determined and plotted as fold induction over vehicle control (dotted line). Data represent the mean ±SEM of three different experiments graphed as fold induction over time-matched vehicle-treated control cells.