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Differentiation of Human Embryonic Stem Cells to Regional Specific Neural Precursors in Chemically Defined Medium Conditions

Figure 5

Neuronal excitability and study of voltage-dependent channels in the newly generated neurons by bFGF and RA treatment.

(A) Action potentials evoked by depolarizing current steps (40 pA) in two different neurons resulted in different firing patterns. In both cases, the spikes were fully blocked with 1 µM TTX (red). (B) Family of voltage-dependent currents obtained from a –30 mV to +50 mV in 10 mV increasing steps protocol from a Vh = −70 mV. An early inward current suggests the presence of voltage-dependent sodium channels, while the second outward component is consistent with the presence of voltage-dependent K+ channels, as seen in the I-V relation. (C) Blockade of TTX-sensitive voltage-dependent Na+ channel. The early component present in (B) was fully blocked with 1 µM TTX (red trace) (n = 39). (D) The outward current was blocked by 2 mM 4-AP (red trace) (n = 10).

Figure 5

doi: https://doi.org/10.1371/journal.pone.0002122.g005