The Chromatin Remodelling Factor dATRX Is Involved in Heterochromatin Formation
Figure 7
Polytene chromosomes show overlap of dATRX and dHP-1a on chromosome IV and pericentric heterochromatin.
A. Polytene chromosome squashes showing double immunostaining for the indicated proteins show overlap between the dATRX-HA protein (green) and dHP-1 staining (red) on the partially heterochromatic chromosome IV (arrowheads). The chromocentre is shown in the enlargements (inset panels). DAPI = DNA staining. B. As (A) but immunostained for the HA tag (green, dATRX-Long and dATRX-Short) and with α-dATRX-Long antibody (blue). Colocalisation of dATRX-Long and dHP-1a is observed at the chromocentre, which is indicated by arrowheads and shown in the enlargements (inset panels).