Intervening with Urinary Tract Infections Using Anti-Adhesives Based on the Crystal Structure of the FimH–Oligomannose-3 Complex
Figure 2
Crystal structure of FimH in complex with oligomannose-3.
The two FimH RBDs related by non-crystallographic symmetry (green and forest-green, Ig-fold labeling) both bind oligomannose-3 (only one is shown in blue cyan), interconnected via Asp47 to a nickel ion (green cyan). His45 and Asp47 and four waters ligate the nickel ion in an octahedral configuration. Sulphate ions (yellow with red oxygens) stabilize a highly charged crystal packing interface by bridging Val112 and Ser113 with Arg132 from the two crystallographically independent RBDs with Arg92 and Tyr55 from a symmetry-related RBD.