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Modular Mass Spectrometric Tool for Analysis of Composition and Phosphorylation of Protein Complexes

Figure 5

(A) An MS/MS spectrum of a phosphopeptide detected at m/z 2789.245. The loss of 98 Da, which is a specific signature of phosphorylation, is indicated. (B) MS/MS/MS of the fragment that lost 98 Da. The identity of the phosphopeptide and the plausible location of the residue which lost the phosphate group are shown in the panel.

Figure 5

doi: https://doi.org/10.1371/journal.pone.0000358.g005