Adaptation of HIV-1 Depends on the Host-Cell Environment
Figure 1
Change in relative fitness of viruses cultured on two distinct T-cell lines.
A) The 6 wildtype replicates that are cultured on MT4 T-cells show a linear increase in fitness. The inset shows a single representative example of replicate wt-1. B) The 6 mutant MT4 cultured replicates show an initial fitness increase that seems to reach a maximum at around a relative fitness of 1.04±0.02. All trajectories are best described by an exponential regression model with an upper limit (F-test, p<0.05). The inset shows a single representative example of replicate mt-1 (see Table 2 for relative fitness values at day 110). C and D) relative fitness of 6 wt and 6 mt C8166 cultured viruses, respectively. Relative fitness did not significantly deviate from the starting virus over the course of the experiment (ANOVA, p>>0.05; see Table 3 for relative fitness values at day 110). Fitness is relative to reference strain v13 (also see materials and methods section.