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The PDZ Protein Canoe/AF-6 Links Ras-MAPK, Notch and Wingless/Wnt Signaling Pathways by Directly Interacting with Ras, Notch and Dishevelled

Figure 3

cno interacts genetically with N, Ras and Wg signaling pathways.

(A–J and P–T) Lateral views of stage 11 embryos stained with an α-Eve antibody.

(B, G) Extra Eve⁺ progenitors are detected both in cno²/Dl^X transheterozygotes and in cno²/cno² , Dl^X embryos.

(C, H) Both cno²/pnt ^Δ88 double heterozygotes and cno², pnt ^Δ88/cno² embryos show loss of Eve⁺ progenitors.

(E, J) An increase in Eve⁺ progenitors is observed both in cno² /aos ^Δ7 transheterozygotes and in cno² , aos ^Δ7 /cno² embryos [74].

(D, I) Gain and loss of Eve⁺ progenitors are detected both in wg^CX4/+; cno²/+ double heterozygotes and in wg^CX4/+; cno^mis1/cno² embryos. (F–I, K–N, P–S): (P) Simultaneous mesodermal overexpression of N^Act-Wg-Ras^Act, (Q) Wg-Ras^Act, (R) N^Act-Wg and (S) N^Act-Ras^Act have similar phenotypes than cno²/cno² (F, K), cno²/cno² , Dl^X (G, L), cno²/cno² , pnt ^Δ88 (H, M) and wg^CX4/+; cno^mis1/cno² (I, N) embryos, respectively.

Arrows and arrowheads indicate gain and loss of progenitors, respectively.

(K–O) Diagrammatic representation of the effect on Eve expression of cno lof (K) and cno lof in combination with reduced doses of N (L), Ras (M) and Wg (N) signaling components, or with increased doses of Ras signal (O).

(T) The phenotype of Ras1^Act mesodermal overexpression is enhanced in a cno² mutant background.

(U) Table shows % of embryos of the indicated genotype that display a wildtype number of Eve⁺ progenitors, Eve⁺ progenitors gain, loss and both (gain and loss).

Legends for bars are shown right to the table.

At least 70 embryos of each genotype were counted.

P<0.0001 for all genotypes except for cno²/cno² , aos ^Δ7 (P = 0.0239) and wg^CX4 /+; cno² /cno^mis1 (P = 0.006).

doi: https://doi.org/10.1371/journal.pone.0000066.g003