Larval crowding enhances dengue virus loads in Aedes aegypti, a relationship that might increase transmission in urban environments

Background Climate change and urbanization will alter the global distribution of disease vectors, changing the disease burden in yet unpredictable ways. Aedes aegypti is a mosquito responsible for transmitting dengue, Zika, chikungunya, and yellow fever viruses that breeds in containers associated with urban environments. We sought to understand how ambient temperature and larval densities in the immature aquatic phases determine adult life history traits and dengue virus loads post-infection. We predicted that larval crowding and high temperatures would both lead to smaller mosquitoes that might struggle to invest in an immune response and, hence, would exhibit high viral loads. Methods We first examined larval densities from urban and rural areas via a meta-analysis. We then used these data to inform a laboratory-based 2x2 design examining the interacting effects of temperature (21 vs. 26°C) and density (0.2 vs. 0.4 larvae/mL) on adult life history and dengue virus loads. Results We found that urban areas had an ~8-fold increase in larval densities compared to more rural sites. In the lab, we found that crowding had more impact on mosquito traits than temperature. Crowding led to slower development, smaller mosquitoes, less survival, lower fecundity, and higher viral loads, as predicted. The higher temperature led to faster development, reduced fecundity, and lower viral loads. The virus-reducing effect of higher temperature rearing was, however, overwhelmed by the impact of larval crowding when both factors were present. Conclusions These data reveal complex interactions between the environmental effects experienced by immature mosquitoes and adult traits. They especially highlight the importance of crowding with respect to adult viral loads. Together, these data suggest that urban environments might enhance dengue virus loads and, therefore, possibly transmission, a concerning result given the increasing rates of urbanization globally.


Introduction
Mosquito-borne diseases like dengue fever [1], chikungunya [2,3], and Zika [4] are rising globally due in part to the expanded range of their vectors, Aedes aegypti and Aedes albopictus.By 2050, half of the world's population will likely live in association with one of these species [5].Ae. aegypti, is especially anthropophilic, thriving in urban areas, where it breeds in artificial containers (buckets, plant trays, air conditioning units, etc.) inside and near human dwellings (reviewed in [6]).The combination of increased urbanization, human-meditated dispersal, and climate change is expanding mosquito ranges [5].The implications of these expansions for virus transmission and human disease burdens are not fully understood [5,7,8] but will necessitate shifts in local needs for vector control and pathogen-specific vaccine and diagnostic deployment.
For organisms with complex life cycles, like mosquitoes, the conditions experienced in the larval phase can carry-over to affect the condition and performance (i.e. the phenotype) of the adult.These phenotypic links between life history stages can be as important as demographic links, shaping the dynamics of the adult populations and, in the case of vectors, the disease burdens they impose.The two most critical components of the larval environment are temperature and larval density.As ectotherms, temperature is a fundamental modifier of mosquito physiology, with higher temperatures speeding larval development rates and reducing adult size [9].Similarly, high larval densities reduce nutrient availability in larval habitats (typically artificial containers) that are already frequently nutrient-poor [10], extending development and, again, reducing adult size.Regardless of the stressor, small adults exhibit reduced fecundity and survival with concomitant effects on vector dynamics [11][12][13].
With respect to the ability of a mosquito to transmit viruses (vector competence), stress experienced in the larval stage matters.On one hand, smaller females, resulting from low larval nutrition, may seek more repeated bloodmeals in the field with a greater cumulative lifetime potential for pathogen transmission [14][15][16].On the other hand, larger females may consume more virus during a single blood meal, increasing their probability of infection [17].For Ae. aegypti, nutritional stress in either mothers or their offspring makes them more likely to transmit Zika virus after a single feeding event [18], exhibiting higher viral loads in their saliva.[19].For dengue virus, the consequences of the larval environment for transmission are unclear, with different studies making different conclusions (Reviewed in [20,21]).There are several possible explanations as to why nutrition may affect viral transmission.Smaller mosquitoes may have fewer excess resources to spend on mounting a costly immune response [22] that would otherwise slow its progression in the mosquito body or reduce salivary viral loads.Starvation may also affect other aspects of physiology, including the permeability of known barriers to tissue progression for viruses like those in the midgut and the salivary glands [18].
Humans are altering the habitats of Aedes via urbanization and global warming [5], both of which are increasing the temperature Aedes experience as larvae.Urban heat island effects [23] will likely exacerbate longer-term temperature increases due to climate change.But urbanization could also alter the larval habitats of Aedes in more subtle ways, driving changes in larval densities.Urbanized environments may be more degraded, such that the abundance of larval predators (birds, beetles, and heterospecific mosquito larvae) is decreased and/or their composition altered (reviewed in [24]).If urbanized environments release Aedes from larval predation, then we might expect systematic differences in larval densities between urban and non-urban habitats [25,26] but formal meta-analyses of the effects of urbanization are lacking.Such differences in larval densities could alter adult phenotypes and affect their vector competence for viruses-such that urban environments enhance infectivity in ways that have not yet been appreciated.Recently generated predictive maps of Aedes mosquito distributions [5,27] and pathogen transmission zones [7,8] have been constructed based on evidence of past field collection of mosquitoes, pathogen transmission zones, laboratory measures of thermal performance for viruses and vectors, and abiotic habitat characteristics.These modeling approaches do not yet account for how stressors, specifically in the larval phase, alter pathogen transmission in adults.
Here, we first explore the evidence for differences in larval densities in urban vs. rural habitats using a meta-analysis of the literature.Any such differences, in combination with cities acting as heat sinks, would have potential consequences for adult vector competence along ruralurban gradients.We then carried out a two x two laboratory-based design to test the effects of temperature and Ae.aegypti density in the larval phase on mosquito life history traits and subsequent adult suitability for dengue virus replication.What is novel in our design is that we have isolated temperature effects in the larvae from those in the adult which confound virus replication.We predicted that higher temperatures and crowding would both produce smaller adult mosquitoes, albeit possibly through two different mechanisms-faster development and stress, respectively.We predicted that the resulting smaller mosquitoes might exhibit reduced survival and fecundity and would invest relatively less energy in immunity, exhibiting higher viral loads.We expected mosquitoes experiencing both crowding and higher temperatures would be more severely affected than mosquitoes experiencing only one of these conditions.

Systematic meta-analysis on mosquito density
To determine whether urban environments harbor higher densities of Aedes larvae than nonurban environments, we conducted a systematic meta-analysis.On March 7 th, 2023, we followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines [28], and searched the peer-reviewed database, Web of Science-Core Collection, for studies comparing Aedes mosquito larval density in urban and non-urban environments (S1 Fig) .Using the search term (urban* "aedes" aegypti or albopictus larval density), we found 2,112 references to be screened at the title and abstract level.Of the 2,112 references, 526 were subject to full-text screening, 67 met most of our inclusion criteria but failed to report the volume of the containers, and 21 met all of our criteria (S1 Table ).Both measures are useful for comparing densities in a relative sense, but only the latter can be used to compare densities in an absolute sense.
To be included in our meta-analysis, studies had to 1) report larval densities (or immature stages, such as eggs or pupae) of 2) Aedes aegypti and/or Ae. albopictus 3) in an urban and non-urban environment 4) on a per-container basis.Non-urban environments included rural, suburban, peri-urban, forest, or industrial environments.Studies that did not classify their sites as urban and non-urban were still included if they reported the human population density of their sites; here, we compared the most populated area to the least populated area.We also included studies that reported mosquito larval density some distance from an urban area; in these studies, we compared the closest and furthest locations.
We excluded studies that did not meet the above inclusion criteria and those that confounded habitat type and urban classification.For example, we would exclude a study that surveyed larvae in tires in the urban environment but tree holes in the non-urban environment.We also excluded studies that compared areas based on socioeconomic status alone and studies that experimentally manipulated mosquito densities.
For each urban-non-urban comparison, we recorded the mean larval density, error associated with the mean, and sample size.Data were extracted directly from the text or tables or from figures using WebPlotDigitizer (version 4.6) [29].From each study, we recorded the mosquito species (i.e., Ae. aegypti and/or Ae. albopictus), how larval density was measured (i.e., larvae per liter, eggs per trap), and the urban-non-urban comparison (i.e., urban-rural, urban-suburban, etc.).We gave each study a unique identifier, as multiple effect sizes came from the same study.
We used the log response ratio to determine whether urban environments have higher densities of Aedes larvae.The log response ratio is calculated as: where X U is the larval density in the urban environment, and X NU is the larval density in the non-urban environment (n effect sizes = 105, n studies = 21).We calculated the unbiased, standardized mean difference, Hedges' g, as per Borenstein et al [30], when studies reported the mean, standard deviation, and sample size (n effect sizes = 202, n studies = 67) but did not include a volume for the larval container, and present this in the supplementary material (S2 Fig) .Hence, we analyzed the 21-study dataset using log response ratios and the 67-study dataset using Hedges' g.We did not analyze Hedges' g for the smaller dataset as there were too few studies that reported the necessary components (n = 11).
Hedges' g and the log response ratio are interpreted as follows: a value of zero means that there is no difference in mosquito density between urban and non-urban environments.A positive value indicates that larval density is greater in urban environments than non-urban environments, while a negative value indicates the opposite.

Larval rearing and 2x2 experimental design
Mosquitoes were derived from a lab-established colony of wildtype Ae. aegypti mosquitoes previously collected from the field in Me ´rida, Mexico, by Pablo Manrique-Saide.Eggs were hatched synchronously in separate trays containing filtered, dechlorinated, and oxygendepleted water for 1h without a food source.After eclosion, first-instar larvae were transferred to 150mL clear hard plastic tumbler cups (6 x 4.5 x 7 cm) containing 100mL of filtered, dechlorinated water, according to their respective density.Larvae were sorted following a 2x2 design ( relative humidity).Twenty larvae/cup is our laboratory's standard low-density rearing regime that minimizes competition and maximizes adult mosquito size.The number of larvae in the crowded treatment was determined through piloting to find a density that reduced mosquito size but did not extend mean development time >7 days beyond that of low-density treatment to allow comparisons under similar environmental conditions/time windows.The two temperatures were selected to represent the average annual low temperature in Merida ´and the optimal temperature for Ae.aegypti [31].Rather than test a third treatment level, such as an average of the upper temperatures in Me ´rida, we opted to expend the experimental effort to test and replicate each condition across a second incubator to reduce any spurious incubator effects that can occur.Regardless of density or temperature treatment, each larval rearing cup was fed approximately 5 mg of ground fish food (Tetramin) daily, which did not vary.This feeding regime (or 0.25 mg food/larva/day) was previously determined as 'low-stress' for 20 larvae or the equivalent of our low crowding treatment, as per [32,33].Our crowded treatment (40 larvae), therefore, received 0.125 mg/larva/day.The previous study employed 0.05 mg/ larva/day to represent extreme stress [33], although this lengthened the development time significantly (>20 days).

Larval development time and survival rate
Larval development, recorded as the day of pupation per individual, was noted daily.Pupae were sexed on emergence by visual analysis of the last segment of their Terminalia using a stereomicroscope.Male and female numbers were recorded, and individuals were placed in cardboard cups (10 x 7.6 x 10cm) covered with mesh.A 10% sucrose solution was offered ad libitum and changed daily to avoid mold.Survival rate was calculated as the number of individuals that pupated out of the total number placed in the cup (i.e.20 larvae/cup).

Fecundity and weight measurement
Pupae from each of the 20 cups were then transferred to individual cages (10 x 7.6 x 10cm) for emergence and placed at 26˚C.All conditions were reared at the same temperature during adulthood.Following mating in the small populations, 5-7 day-old females were offered a bloodmeal of blood from anonymous human donors (BioIVT) using a Hemotek membrane feeding system (Hemotek).Females were deprived of sucrose 12 hours before feeding.Given the differences in developmental rate imposed by the 2x2 regime described above, feeding was first offered to all females from the density of 20 larvae/cup (both temperatures).One week later, females from the 40 larvae/cup density were fed using the same procedure and blood donor.Forty-eight hours post bloodmeal, a total of six females per each one of the twenty cages (120 fecundity measures per treatment) were placed individually in 70mL oviposition cups containing moist filter paper, with access to 10% sucrose to estimate fecundity.Females were allowed to lay eggs for three consecutive days.On the third day, egg papers were collected and manually counted using a stereomicroscope.After collecting egg papers, females were starved for 48-56h, anesthetized, and individually weighed live using an analytical balance.

Vector competence
To assess the impact of temperature and crowding on mosquito vector competence, we ran a parallel but separate 2x2 design consisting of the same design described above but reducing the number of replicates from twenty to ten.At 5-7 days of adulthood, females were offered an infectious blood meal containing the DENV serotype 2 strain ET300 (GenBank: EF440433).The schedule of blood feeding was the same as above for the fecundity assessment, with the density of 20 larvae/cup feeding a week earlier than females from the 40 larvae/cup treatment.
Mosquitoes were offered blood from the same blood donor.Additionally, to avoid using a frozen virus with less activity in both feeding schedules, mosquitoes were offered a freshly harvested normalized viral titer of 3 x 10 7 viral gene copies/mL per feed (as per below).Fed females were then housed in their respective cages, according to the 2x2 design, until collection.At 14 days post-viral feed, individual females had their legs dissected and stored in 300μL of Trizol (Sigma), then transferred to -80˚C until further processing.This collection was done to assess virus transmission potential, as recently shown to be a more accurate method than saliva collection through the capillary method [34].

Virus culture and quantification
The virus was cultured in Ae. albopictus C6/36 cells at 26˚C in RPMI 1640 medium (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS) and HEPES buffer (20mM final concentration).Cells were first allowed to form monolayers of around 60-80% confluence in T-175 flasks (Sigma Aldrich, St. Louis, MO) and then were inoculated with DENV and maintained in RPMI medium supplemented with 2% FBS.At day 7 post-inoculation, live virus was harvested, titrated via absolute quantification RT-qPCR, and adjusted to a final viral load of 10 7 DENV copies per mL.
All material for virus quantification (supernatant, tissues) was placed in 300 μL of Trizol (Invitrogen) in 1.5 mL microfuge tubes (Sarstedt, Nu ¨mbrecht, Germany) and extracted as per the manufacturer's instructions.A 2.8 mm ceramic bead was added to tissue dissections first, and samples were homogenized on a Bead Ruptor Elite (Omni International, USA) and then frozen at −80˚C.RNA was resuspended in 50 μL nuclease-free water and quantified using the NanoDrop 2000 spectrophotometer system (ThermoFisher Scientific).RNA was then treated with 5 units of DNase I (Sigma-Aldrich) at room temperature for 15 min, followed by inactivation with 50 mM EDTA at 70˚C for 10 min and stored at -80˚C.
DENV virus was quantified using TaqMan Fast Virus 1-step Master Mix (Thermo Fisher Scientific) in 10-μL reaction volumes with DENV-specific primers and probes as per previous [35].The following protocol was used: reverse transcription at 50˚C for 5 min, reverse transcription inactivation at 95˚C for 20 sec, followed by 50 amplification cycles of denaturation at 95˚C for 30 sec and priming/extension at 60˚C for 30 sec.A standard reference curve of known quantities of a DENV-2 genomic fragment was used for absolute quantification by qPCR.The DENV-2 genomic fragment was previously [35] inserted into a plasmid and transformed into Escherichia coli as described.The linearized and purified fragment was serially diluted, ranging from 2.5x10 7 to 2.5x10 3 copies μL, and used to create a standard curve of DENV amplification.The standard curve was run in duplicate on each 96-well plate, and the limit of detection was set at 10 2 copies.All samples were run in triplicate.

Statistical analysis
For the meta-analysis, we used a mixed-effects model to determine whether larval density was greater in urban or non-urban environments.We ran two models, one with the log response ratio as the response and one with Hedges' g as the response.Our models included an intercept and study as a random effect-as multiple effect sizes came from the same study.Including 'study' as a random effect accounted for 41% of the variance in the log response ratio model and 17% of the Hedges' g model.We checked for publication bias by examining funnel plots of the sample size and effect sizes for the log response ratio and of the precision and model residuals for Hedges' g.The funnel plots were uniformly distributed, indicating no evidence of publication bias (Figs 2 and S2).
We used a mixed-effect model for all variables where Incubation Temperature and Larval Density were fixed effects, Incubator(Temperature) was a random effect, and Experimental Run was a fixed blocking effect.Note that the design was partly nested where different factors are tested over different denominators depending on the scale at which the treatment is applied.Temperature effect were generally tested over the denominator of Incubator(Temperature), density effects were tested of Cup(Temperature x Density), see Quinn and Keough (2002) for further details [36].

Results
Based on 21 published studies (S1 Table ), Aedes larval density per container in breeding habitats was higher in urban environments than in non-urban environments (mean Log Response Ratio: 0.832, t 20 = 2.218, p = 0.038, Fig 2).On average, larval densities were 8.6 times (±3 SE) higher in urban environments than non-urban environments.For a broader range of studies, where comparisons were made between urban and rural environments, but container size was not specified, we found similar effects based on Hedges' g (mean Hedges' g: 0.228, t 66 = 2.07, https://doi.org/10.1371/journal.pntd.0012482.g002P = 0.042).Excluding the most extreme values, the mean densities across the 21 studies were 0.5 larva/mL and 1 larva/mL, for rural and urban, respectively.We saw crowding effects on viral load in our design (above) under less extreme conditions: 0.2 larvae (low density) and 0.4 larvae (high density).In most of the published studies, collections were from tropical or subtropical regions, and while temperature during collections (often over many weeks) was not always reported, the local annual average low to average highs for each ranged from 21-36˚C.
To then test the relative importance of larval densities and temperature on adult mosquito life history traits and virus transmissibility by the mosquito, we reared larvae at either crowded (40 larvae per cup) or uncrowded (20 larvae per cup) conditions at two temperatures (21˚C or 26˚C) (Fig 1).Given equal amounts of food, crowding equates to reduced access to nutrients for the higher-density group.We then measured a range of adult traits, including pupation time, sex ratio, weight, fecundity, survival, and vector competence for dengue virus.
All adult female mosquitoes were infected with DENV (100% prevalence in all treatments) when feeding on infectious blood.Total body viral loads, our measure of vector competence, however, varied (Fig 4) and exhibited a somewhat bimodal distribution, which is common to such studies [37].Larval density (F 1, 36 = 7.94, p = 0.008) and the interaction with temperature (F 1, 36 = 5.91, p = 0.020) were significant.The main effect of temperature was not (F 1,2 = 15.73,p = 0.058).High-temperature rearing decreased total viral loads in adults on average 4-fold in mosquitoes reared at lower densities and by only 1.2-fold for mosquitoes reared at high densities.In short, the lower temperature and crowding condition increased virus replication, whereas the higher temperature with reduced crowding had the opposite effect.The findings were not as predicted, with the high temperature and crowding conditions acting in opposition, indicating that virus loads cannot be driven purely by body size.
As expected, crowding negatively affected most traits (slower development, reduced female survival, smaller mosquitoes, reduced fecundity, higher viral loads).Mosquitoes reared at the higher temperature did develop more quickly but exhibited fewer other effects except for fecundity, making crowding a more impactful trait determinant.This also held true for viral loads, where even when the higher temperature led to reduced loads, the effect was largely erased by crowded rearing.

Discussion
Our meta-analysis indicated that urban environments tend to increase larval densities in breeding habitats (by at least 8-fold).Our laboratory work then demonstrated that larval density effects affected all the adult traits we studied and, in some cases, modified the effects of temperature.Larval crowding reduced female survival at the pupal stage, slowed development, and produced smaller adults with reduced fecundity and longevity.Most importantly, we found that larval crowding increased viral loads.The higher temperature had weaker effects than anticipated, speeding development and reducing fecundity but did not produce smaller mosquitoes, although there was a trend in that direction.The higher temperature also resulted in lower disseminated viral loads, counter to expectation, but this only remained true in the absence of larval crowding.
We suspect nutrient limitation under crowding drives the effects of larval crowding on vector competence.Individuals who experience resource limitation in the larval phase may imbibe less blood per sitting [17] or feed more frequently [16] as adults and may also have thinner midgut basal lamina [18].This tissue barrier limits the dissemination of viruses into the body, and thinning via resource limitation is associated with greater dissemination and higher viral loads in the saliva, which may translate to greater transmission.Larvae with restricted intakes also exhibit changes to their bacterial microbiomes in the adult stage, including lower total abundance and reduced diversity [12].The microbiome can affect vector competence for viruses [38] via innate immune system activation, direct antiviral action, or resource competition [39].Larval crowding alters myriad other factors beyond resource intake, many of which could contribute to viral loads.For example, crowding has been shown to affect gene expression in the fat body-a metabolic and fat-storage organ.Of the many pathways whose expression is affected, those involving immunity and autophagy may directly affect viral replication [40].
There are two potential larval pathways to reduced adult size-either by faster development in higher temperatures [41] or slower development in crowded conditions, as seen here.The warmer temperature/faster development yielding smaller adult sizes conforms to the 'hotter is smaller' rule common to most animals (reviewed in [42]).In contrast, larvae took longer to develop under crowded conditions but still emerged smaller as adults-an effect that is also well-established in animals [43,44].Of the two factors we manipulated, crowding had a greater effect on the range of life history traits than temperature and was a stronger determinant of developmental rate.While the opposite has been demonstrated elsewhere [41], the level of crowding may be the greater stressor in our study, with our 26˚C degree treatment being a temperature routinely experienced by Ae. aegypti in its tropical range [5,27] and in the laboratory.We expect exposure to a slightly higher temperature or simply greater replication to enhance power across our study design, which might have yielded a significant relationship between high temperature and reduced size, given the estimates seen here (p = 0.071).
For most animal species, urbanization poses challenges.As cities expand and infrastructure develops, natural habitats are fragmented and destroyed, resulting in the loss of critical habitats [45].Some species adapt to urban environments, thriving in human-altered landscapes and taking advantage of novel food sources and habitats [45,46], while others struggle to adapt, facing population declines and even extinction [47].Ae. aegypti is an example of the former, a highly adapted 'human habitat specialist' breeding in artificial containers [6] and feeding almost exclusively on humans [48].The higher larval densities in cities demonstrated by our analysis may be driven by reductions in predators/predator diversity [24], or more frequent access to nutrient-contaminated plastic trash as breeding sites [49], but could also result from the greater reproductive success of females with higher accessibility to hosts [50].Regardless of the mechanisms, the difference in larval densities between rural and urban densities is remarkably high and may drive increases in viral transmission replication and or transmission potential.
There are several limitations to our study.First, temperature typically has curvilinear effects on life history traits and vector competence traits, exhibiting an optimum, with a poorer performance at higher and lower temperatures [51].We used only two temperatures because we were interested in exploring the potential synergies between temperature and larval density in a well-replicated design across multiple incubators.Future studies could explore additional temperatures, especially in the warmer ranges, given Ae.aegypti's thermal preferences and geographic distribution.In contrast, reduced low resource supply in the larval phase will likely negatively affect vector fitness across all temperatures [52].Second, the viral load we used in the blood meal produced 100% prevalence in all mosquitoes by the 14-day post-infection assay point, but surveying earlier time points or feeding a lower initial dose may have revealed differences in the treatments.The range of DENV viral load in human blood varies substantially and can also be several orders of magnitude lower than what was used here [53].We do know from a previous kinetics study where we fed Ae. aegypti with the same virus strain at two different titers (low -10 5 /mL vs. high-10 8 /mL), that the former led to lower viral loads in the midgut and carcass and resulted in lower infection prevalence (70 vs 100% at day 14, respectively) in the salivary glands [37].This suggests that substantial differences in viral load in intermediary tissues also correlate with prevalence or infectivity in the salivary glands, ie vector competence.Additionally, repeated sampling across earlier time points could shed light on both the extrinsic incubation period and early infection prevalence estimates that will be less than zero.Having these two additional parameters, as well as measures of adult survival, would allow for the estimation of vectorial capacity under the Ross-MacDonald equation [54], encompassing some of the intrinsic effects [55] of growth under our temperature x density conditions.Similarly, the additional inclusion of adult survival and egg clutch timing would allow for more holistic interpretations of population growth, or r [56].

Conclusions
With increasing global temperatures and urbanization expected in the future [5,27], temperature and larval density effects will increasingly interact over Ae. aegypti's geographic range.Urban heat sink phenomena will only further exacerbate temperature effects in cities [23].Our findings indicate, however, that while higher temperatures in the larval phase might mitigate viral loads in adults, larval crowding can overwhelm these effects, increasing viral loads and possibly transmissibility.This study illustrates that focusing on single global change stressors in isolation may yield inaccurate predictions of the transmission potential of vectors.

Fig 1 )Fig 1 .
Fig 1. Experimental design for Aedes aegypti exposure to temperature and crowding effects (2x2) with replication across incubators in the larval phase followed by measurement of adult life history traits as well as vector competence for dengue virus.https://doi.org/10.1371/journal.pntd.0012482.g001

Fig 2 .
Fig 2. Summary of effect sizes of meta-analysis of the effects of urbanization on larval density in disease vectors.Points to the right of zero indicate that urbanization increased density, points to the left indicate that urbanization decreased larval density.The red dotted line indicates the mean effect size, and the dot with whiskers indicates the mean with 95% confidence intervals.The Y axis indicates the sample size of each study on a log10 scale.

Fig 3 .
Fig 3. Life history traits for two larval rearing temperatures (blue = 21˚C, red = 26˚C) and two larval densities (20 vs. 40 individuals).(A) Egg to pupal stage development time in days reported as time to 50% hatch n = 20 per treatment.(B) Survival percentage to the pupal stage.n = 60 per treatment.(C) Adult female total body weight (mg) post 48 hours starvation.N = 20 per treatment.(D) Adult female fecundity, as measured by total eggs produced over three days post blood feeding, is n = 20 per treatment.In all cases, individuals are shown in the figure, but the analysis reflected the number of independent applications of each treatment at the level of cups.The mean is denoted with a bold data marker with a line through it.https://doi.org/10.1371/journal.pntd.0012482.g003

Fig 4 .
Fig 4. Adult female disseminated viral loads.Dengue virus copy number in leg tissues as determined by qRT-PCR relative to a standard curve and reported per 125 ng RNA for two larval rearing temperatures (blue = 21˚C, red = 26˚C) and two larval densities (20 vs. 40 individuals).n = 10 per treatment.Individuals are shown in the figure, but the analysis reflected the number of independent applications of each treatment at the level of cups.The mean is denoted with a bold data marker with a line through it.https://doi.org/10.1371/journal.pntd.0012482.g004