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A pilot study demonstrating the identification of Trypanosoma brucei gambiense and T. b. rhodesiense in vectors using a multiplexed high-resolution melt qPCR

Fig 2

Melt profile of field samples.

Three samples were identified as T. b. rhodesiense due to the production of both PLC and TBR peaks (samples:1027, 1079 and 1137). Samples which only produced PLC peaks were considered to be T. b. brucei (samples: 2362,2377 and 2380). The TBR positive control was T. b. rhodesiense DNA and the negative control used was nuclease free water. Normalized fluorescence dF/dt is plotted against degrees °C (deg.) with threshold indicated in red and species-specific bins shown in grey.

Fig 2

doi: https://doi.org/10.1371/journal.pntd.0008308.g002