Epigenetic regulation of defense genes by histone deacetylase1 in human cell line-derived macrophages promotes intracellular survival of Leishmania donovani
Fig 6
HDAC1 inhibiting drug SAHA impairs parasite survival.
THP-1 cells (105 cells/ml) were infected with L. donovani (20:1 MOI) for 3 h.The infected (IN) cells were then incubated with increasing amounts of SAHA and were then harvested at 6 h. A: Expression of HDAC1 infected (IN) THP-1 cells after treatment with different concentrations of SAHA. Significance was measured concerning infected and untreated samples (SAHA concentration– 0 uM). B: Dose-dependent impact of HDAC1 inhibitor, SAHA, on the parasitemia count. Infected THP1 cells (IN) were incubated with increasing concentrations of SAHA for 6 h. Cells were stained after 6 h and amastigotes enumerated visually. C: The expression levels of the host defense genes, 6 h post-infection in the absence and presence of SAHA (5 μM) were quantified by qRT-PCR. Results represent the mean ± SD (n = 3). The statistical significance was determined using ANOVA, ns (P>0.05), * (P≤0.05), ** (P≤0.01), *** (P≤0.001), *** (P≤0.0001) and **** (P≤0.00001).