Design of multi-epitope peptides containing HLA class-I and class-II-restricted epitopes derived from immunogenic Leishmania proteins, and evaluation of CD4+ and CD8+ T cell responses induced in cured cutaneous leishmaniasis subjects
Fig 4
IL-10 responses to multi-epitope peptides used individually or as pools composed of HLA-I or HLA-II peptides.
IL-10 was quantified by ELISA, in culture supernatants of PBMC from cured CL subjects (N = 16) or healthy individuals (N = 10) stimulated with multi-epitope peptides (1 μM) used individually (A), or as peptide pools composed of HLA-I (B) or HLA-II (C) peptides, for 10 days. PHA and SLA were used as positive controls (10 μg/ml). Horizontal bars represent median values. Statistical significance was assigned to a value of p<0,05 (*p<0.05, **p<0.01, ***p<0.001).