Vaccination with single plasmid DNA encoding IL-12 and antigens of severe fever with thrombocytopenia syndrome virus elicits complete protection in IFNAR knockout mice
Fig 2
Characterization of gene expression after SFTSV DNA vaccine transfection.
(A) Expression and secretion of the viral antigens and IL-12 in HEK293 cells transfected with the DNAs was confirmed by measuring the concentration of Flt3L and IL-12 in the cell culture supernatants by ELISA. Data are presented as mean + S.D. from triplicated experiments. (B) Expression of the viral antigens, Gn (~ 74 kDa), Gc (~ 71 kDa), and NP-NS fusion (~ 80 kDa) proteins, in cell lysates (left panels) and culture supernatants (right panels) of transfected HEK293 cells was assessed by immunoblot analysis using anti-Gn, Gc, or NP antibodies, respectively. The specific bands of antigens corresponding to their expected sizes are indicated with arrow heads. β-actin was used as loading control.