Identification of a systemic interferon-γ inducible antimicrobial gene signature in leprosy patients undergoing reversal reaction
Fig 6
GBPs are upregulated in the peripheral blood of leprosy patients during RR.
(A) Heatmap of GBPs 1 to 6 normalized counts from all 10 RR temporal stages (BR; RR and AT). Significance was determined by paired one-tailed ANOVA and post-hoc (Tukey multiple comparison test). (B) GBPs 1 to 6 correlation of fold change (FC) and p-value (-log p-value) between RNAseq and qPCR data. Squares represent the mean FC (RNAseq and qPCR) of the 10 RR pairs (RR vs. BR). Pearson correlation was used to calculate r values and the two-tailed p-value was calculated for correlation significance. (C) Distribution of GBPs 1 to 6 normalized counts in the whole blood for all groups of leprosy patients. The graph shows the mean per clinical type. MB = 8, BR = 10, RR = 10, AT = 10 and PB = 8 (D) Distribution of GBP 1 to 6 mRNA by microarray analysis of skin lesions shown in arbitrary units (AU). The graph shows the mean per clinical type. L-lep/MB = 6; RR = 7 and T-lep/MB = 10. Significance was determined by one-tailed ANOVA using GraphPad Prism software and post-hoc (Tukey multiple comparison test). (E) Distribution of GBPs 1 to 6 mRNA in the RNAseq analysis of human MDMs stimulated for 24h, media, yellow; IFN-β, red; IFN-γ, blue and TLR2/1 ligand, green. The graph normalized counts for all 5 individuals per stimuli for GBPs 1 to 6. Significance was determined by paired one-tailed ANOVA using GraphPad Prism software and post-hoc (Tukey multiple comparison test). * P <0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. MB = multibacillary, BR = before reaction, RR = reversal reaction, AT = after treatment, PB = paucibacillary, L-lep = lepromatous leprosy and T-lep = tuberculoid leprosy.