Development of a toolkit for piggyBac-mediated integrative transfection of the human filarial parasite Brugia malayi
Fig 1
Maps of plasmid constructs used in this study.
ITR = inverted terminal repeat; HPRO = BmHSP70 promoter; GX1 = Gaussia luciferase ORF exon 1; HINT = BmHSP70 intron 1; GX2 = Gaussia luciferase ORF exon 2; HUTR = BmHSP70 3' UTR; MCS = multiple cloning site (Bcl 1—Bgl II—Mlu 1—Cla 1—Sma 1—Aat II—Nde I—Bcl II); TRASNP = piggyBac transposase ORF; RPRO = Bmrps12 promoter; RUTR = Bmrps12 3' UTR; GFP = Green Fluorescent Protein ORF; YFP = Yellow Fluorescent Protein ORF; CHR = Cherry Red ORF.