Benzoxaborole treatment perturbs S-adenosyl-L-methionine metabolism in Trypanosoma brucei
Fig 3
Comparative analyses of T. b. brucei treated with AN5568 and sinefungin, a non-specific methyltransferase inhibitor.
A) A fixed-ratio isobologram analysis was conducted using sinefungin and AN5568. B) Comparative metabolomics analysis was carried out to assess any common metabolic alterations between sinefungin- and AN5568-treated cells. A principal component analysis (PCA) plot of the 3 sample group was generated. C) Individual metabolites were compared in terms of their fold change over a control group. D) AN5568 treatment leads to increased abundance in components of the trypanothione biosynthesis pathway, whilst sinefungin treatment does not.