The novel nematicide wact-86 interacts with aldicarb to kill nematodes
Fig 2
Wact-86 resistant mutants harbour missense mutations in the carboxylesterase gene ges-1.
(A) wact-86 dose-response assays for wild-type worms and the three wact-86 resistant mutants. For each resistant strain the ges-1 allele and the GES-1 amino acid substitution are indicated (see S2 File for the whole genome sequencing data obtained for the resistant mutants). (B) Sequence alignment of the C. elegans GES-1 protein with the orthologous carboxylesterases from fly, fish, mouse, and human. For clarity, only the segment that is mutated in the wact-86 resistant strains is shown. Conserved residues are highlighted in black. The two GES-1 residues that are mutated in the wact-86 resistant strains are highlighted in grey. The asterisk denotes the conserved histidine that is part of the enzyme’s catalytic triad.