Identification of three immunodominant motifs with atypical isotype profile scattered over the Onchocerca volvulus proteome
Fig 4
Reactivity in peptide ELISA can be inhibited by peptides with the same motif.
Reduction in titer was determined by addition of non-biotinylated peptide to 2 different samples during incubation on the ELISA plates. Plates are coated with the biotinylated peptide as indicated in the x-axis. Titration curves were determined (S3 Fig) and titer was calculated as the dilution showing an Abs405nm that corresponds to 10-fold reduction in absorbance of the non-inhibited sample at the start dilution (i.e. 200-fold). As a negative control, the non-relevant peptide JCV_VP2_167-15mer was included. All self-inhibition results are indicated with *.