Impact of CD4+ T Cell Responses on Clinical Outcome following Oral Administration of Wild-Type Enterotoxigenic Escherichia coli in Humans
Fig 4
Percentage of pTfh and cytokine production by pTfh in Resistant and Susceptible volunteers.
A) Live single cell CD14- CD19- events were gated on CD3, followed by gating for CD4 and then CXCR5. The percentage of CD4 cells positive for CXCR5 is indicated at baseline (D -7) and 3 days post challenge (D 3). B) Net CFA/I-specific TNF-α production above pre-challenge (D -7) by CD4+ CXCR5+ (pTfh) on day 3 post-challenge (D3). C) Net CFA/I-specific IL-2 production above pre-challenge (D -7) levels by CD4+ CXCR5+ (pTfh) on day 3 post-challenge (D3). * p < 0.05 ** p < 0.01