Isothermal Diagnostic Assays for Monitoring Single Nucleotide Polymorphisms in Necator americanus Associated with Benzimidazole Drug Resistance
Fig 6
Evaluation of SmartAmp2 genotyping assays in fecal samples spiked with N. americanus larvae (L3).
SmartAmp2 assay using the wild-type (WT)-primer set on DNA extracted from purified L3 (no feces) (red circle), fecal samples spiked with L3 (blue triangle) and fecal samples without L3 (purple square). Negative controls (NC; no feces, no larvae) were included in each run (green diamond).