Modulation of Hemostatic and Inflammatory Responses by Leptospira Spp.
Fig 3
Leptospires and their secreted products induce pro-coagulant activity in human PBMCs.
Human PBMCs were isolated and incubated with virulent L. interrogans serovar Copenhageni L1-130, culture-attenuated L. interrogans serovar Copenhageni, and saprophytic L. biflexa (panel A and C) or their respective culture supernatants (panel B and D). After incubation, PBMCs were washed and the ability of the cells suspensions to induce clot formation on fresh human plasma was determined by measuring recalcification clotting times in the absence (panel A and B) or presence of the aPTT reagent (panel C and D). Cells incubated in buffer alone or fresh culture medium were used as controls. The bars represent the means ± standard deviation of three replicates and are representative of two independent experiments.