Early Regulation of Profibrotic Genes in Primary Human Cardiac Myocytes by Trypanosoma cruzi
Fig 5
Invasive T. cruzi trypomastigotes up regulate the expression of antifibrotic genes in PHCM.
Serum starved PHCM monolayers exposed to invasive T. cruzi trypomastigotes at different time points were used for evaluating the fibrotic gene transcription profile by PCR array and western blot assays. (a) Total RNA (500ng) purified from the cells were converted to cDNA and used for antifibrotic gene transcript quantitation by fibrosis RT2 Profiler PCR Arrays. Line graph shows the mean±SEM (n = 3) of normalized fold regulation of each of the designated antifibrotic genes at different time points. (b) Cell lysates of PHCM exposed to T. cruzi at different time points were separated by SDS-PAGE, transferred to NC membrane, probed with anti BMP7 antibody and developed by chemiluminescence. Blots showing the bands for BMP7 and β-actin are a representative of three independent experiments conducted with similar results. (C) Column graph summarizes densitometric analysis of 3 independent experiments showing BMP7 protein expression kinetics normalized against β -actin. *P< 0.05 vs controls.