Cellular Growth and Mitochondrial Ultrastructure of Leishmania (Viannia) braziliensis Promastigotes Are Affected by the Iron Chelator 2,2-Dipyridyl
Figure 4
Ultrastructural effects of 2,2-dipyridyl on L. (V.) braziliensis promastigotes.
(A) Untreated parasite presenting typical elongated morphology with normal kinetoplast (K), mitochondrion (M), endoplasmic reticulum (ER) and nucleus (N). (B–E) Promastigotes treated with 100 µM 2,2-dipyridyl revealed marked mitochondrial swelling with loss of cristae and matrix (black asterisks). There were concentric membranar structures inside the organelle (arrows), without any impairment of the classic kDNA arrangement. The treatment with 100 µM 2,2-dipyridyl also induced Golgi disruption (white asterisks) and extensive cytoplasmic vacuolization (V). Bars = 200 nm.