Targeting the Non-structural Protein 1 from Dengue Virus to a Dendritic Cell Population Confers Protective Immunity to Lethal Virus Challenge
Figure 3
Induction of anti-NS1 antibody responses in mice immunized with the fusion αDEC-NS1 and αDCIR2-NS1 mAbs.
(A) Immunization protocol describing the order of immunizations, bleedings and challenge. Mice were immunized i.p. with 2 doses of 5 µg of each fusion antibody in the presence of 50 µg of poly (I:C) or 10 µg of recombinant NS1 co-administered with 50 µg of poly (I:C). (B) Anti-NS1 antibody titers 4 days before (white symbols) or 14 days after (black symbols) the administration of the second dose. (C) Anti-NS1 antibody titers obtained 14 days after the second dose were detected using intact (white symbols) or boiled (black symbols) NS1 protein. Symbols represent individual mice and horizontal lines represent the mean value for each group. Fusion antibodies used for immunizations are depicted below. Data were analyzed by a one-way ANOVA followed by the post-test HSD Tukey. P-value indicators * and *** refer to p<0.05 and p<0.01, respectively, while ns = not significant.