Targeting the Non-structural Protein 1 from Dengue Virus to a Dendritic Cell Population Confers Protective Immunity to Lethal Virus Challenge
Figure 1
Production and characterization of αDEC-NS1 and αDCIR2-NS1 mAbs.
Approximately 1 µg of each mAb was separated under reduced (A) and non-reduced (B) conditions. Both gels were stained with Coomassie Blue dye. The heavy (HC) and light (LC) chains of the following antibodies are shown: 1) αDEC, 2) αDCIR2, 3) αDEC-NS1, 4) αDCIR2-NS1. (C) Western blotting using anti-mouse IgG and anti-mouse kappa chain conjugated to HRP to verify the recognition of both heavy and light chains of each antibody. The numbers indicate the same order as in (A). (D) Western blotting on a non-reduced gel using a mouse serum raised against the dimeric form of NS1 followed by protein A conjugated to HRP. Columns 1–4, same as (A), column 5 contains 1 µg of NS1 produced in bacteria (Di_NS1: dimeric NS1 and Mo_NS1: monomeric NS1). Specific reaction was obtained only in columns containing NS1. MW, molecular weight marker in kDa.