Development of Novel Prime-Boost Strategies Based on a Tri-Gene Fusion Recombinant L. tarentolae Vaccine against Experimental Murine Visceral Leishmaniasis
Figure 3
Cytokine production by splenocytes in vaccinated and control mice.
IFN-γ (panel A), IL-10 (panel B), IFNγ/IL-10 ratio (panel C), IL-2 (panel D) and nitrite (NO2) (panel E) production after stimulation with rA2-rCPA-rCPB (column 1), F/T L. tarentolae A2-CPA-CPB-CTE-EGFP (column 2) and F/T L. infantum WT (column 3) as recall antigens. G1 to G5 groups are as indicated in Figure 2. Each bar represents a mean±S.E. in pg/ml (for all cytokines) or in µm for nitrite. The number of independent repeats was two and all tests were done in triplicates (number of mice per group/time point n = 3) and the results are pooled and shown as mean±S.E. of measures obtained from 6 mice in different groups. The asterisk indicates the significant difference between values at the indicated time points as determined by Student's test (p<0.05 denoted as *, p<0.01 denoted as **, p<0.001 denoted as *** and n.s. denoted as non significant).