Severe Hemorrhagic Fever in Strain 13/N Guinea Pigs Infected with Lujo Virus
Figure 6
A. High-magnification photomicrograph of a liver from a guinea pig infected with LUJV. The center is composed of necrotic hepatocytes admixed with cellular debris, and is surrounded by moderate numbers of histiocytes. Hematoxylin and eosin stain; 200× original magnification; scale bar = 50 µm. B. Low magnification photomicrograph of the interface of normal (N) and infarcted (I) area of the liver from a guinea pig with LUJV infection. The infarcted area is cell-poor, and inflammatory cell reaction (arrow) is observed at the interface. Hematoxylin and eosin stain; 100× original magnification; scale bar = 100 µm. C. High-magnification photomicrograph of the papillary muscles of the heart from a guinea pig with LUJV infection. The muscle fibers (solid arrows) are slightly swollen and hypereosinophilic. Small nests of mononuclear inflammatory cells (dashed arrow) separate the myofibers. Hematoxylin and eosin stain; 200× original magnification; scale bar = 50 µm. D. High magnification photomicrograph of the mesenteric lymph-node from a guinea pig infected with Lujo virus (terminal case). The medullary sinuses are filled with large numbers of erythrocytes and there are scattered macrophages that contain intracytoplasmic erythrocytes (erythrophagocytosis, arrows). This lesion is compatible with a prior hemorrhage in the drainage area of the mesenteric lymph-node. Hematoxylin and eosin stain, 400× original magnification, scale bar = 20 µm. Panels A, B, C, D (wtLUJV) infected.