Genetic and Functional Role of TNF-alpha in the Development Trypanosoma cruzi Infection
Figure 1
Levels of TNF-alpha in culture supernatants.
TNF-alpha was assayed by ELISA in supernatants of PBMC cultured for 48 h in the absence of any stimulus and in the presence of T. cruzi antigen, LPS and PHA. A. Subjects were grouped according to positive or negative serology; *p = 0.0003 (Mann-Whitney test). B. Subjects were grouped according to the cardiac or indeterminate form and negative serology: Cardiac x negative to medium: p = 0.0002; cardiac x negative to LPS stimulation: p = 0.005; indeterminate x negative to PHA: p = 0.03; cardiac x indeterminate to medium: p = 0.01; to LPS: p = 0.005; to T. cruzi antigens: p = 0.01 (Mann-Whitney test). Results are expressed as pg/mL. The horizontal line indicates the median, bars the 25% and 75% percentiles, and vertical lines the 10% and 90% percentiles.